Journal: Journal of Traditional and Complementary Medicine
Article Title: Bushen Huoxue Recipe enhances the immune microenvironment at the maternal-fetal interface via the Hippo signaling pathway in mice with recurrent spontaneous abortion
doi: 10.1016/j.jtcme.2025.02.005
Figure Lengend Snippet: Effect of BSHXR on the Hippo signaling pathway. (A) The expression levels of proteins in the Hippo pathway. (B) Quantification of the protein levels. (p-MST/t-MST: Con vs. RSA, p = 0.0179; RSA vs. CsA, p = 0.8320; RSA vs. BSHXR, p = 0.0450; p-LATS/t-LATS: Con vs. RSA, p = 0.0030; RSA vs. CsA, p = 0.0153; RSA vs. BSHXR, p = 0.0327; p-YAP/t-YAP: Con vs. RSA, p = 0.0131; RSA vs. CsA, p = 0.0432; RSA vs. BSHXR, p = 0.0340; p-TAZ/t-TAZ: Con vs. RSA, p = 0.0045; RSA vs. CsA, p = 0.0437; RSA vs. BSHXR, p = 0.0050). (C) Representative images of immunofluorescence staining for YAP and TAZ in decidual tissues. Scale bar: 20 μm (YAP/TAZ/DCS1). (D) The expression of YAP and TAZ in the nuclear or cytoplasmic fraction. (E) Quantification of the protein levels. (YAP: Con vs. RSA, p = 0.0079; RSA vs. CsA, p = 0.0228; RSA vs. BSHXR, p = 0.0163; TAZ: Con vs. RSA, p = 0.0001; RSA vs. CsA, p = 0.0090; RSA vs. BSHXR, p = 0.0006). The data are expressed as the means ± SEMs. ∗ p < 0.05, ∗∗ p < 0.01 and ∗∗∗ p <0.001, compared with the control group; # p < 0.05, ## p < 0.01 and ### p < 0.001, compared with the RSA group.
Article Snippet: Antibodies against TLR2 (66645-1-Ig), TLR4 (66350-1-Ig), TLR7 (17232-1-AP), and GAPDH (60004-1-Ig) were purchased from Proteintech (Rosemont, IL, USA); antibodies against RORγt (ab207082) and FoxP3 (ab215206) were purchased from Abcam (Cambridge, MA, USA); Alexa Fluor 555 nm (8953) and antibodies against MST (14946), p-MST (49332), LATS (3477S), p-LATS (8654S), YAP (14074S), p-YAP (13008S), TAZ (83669S), and p-TAZ (59971S) were purchased from Cell Signaling Technology (Danvers, MA, USA); and an antibody against Lamin B (A11495) was purchased from ABclonal (Wuhan, China).
Techniques: Expressing, Immunofluorescence, Staining, Control
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